The effect of tetrabromobisphenol A on protamine content and DNA integrity in mouse sperm

0463181 - BTÚ 2017 US eng C - Konferenční příspěvek (zahraniční konf.)
Žatecká, Eva - Castillo, J. - Elzeinová, Fatima - Kubátová, Alena - Děd, Lukáš - Pěknicová, Jana - Oliva, R.
The effect of tetrabromobisphenol A on protamine content and DNA integrity in mouse sperm.
Abstract book of the 48th Annual Meeting of the Society for the Study of Reproduction. San Juan: Society for the Study of Reproduction, 2015.
[48th Annual Meeting of the Society for the Study of reproduction. San Juan (US), 18.06.2015-22.06.2015]
Grant CEP: GA ČR(CZ) GA P503/12/1834; GA MŠMT(CZ) ED1.1.00/02.0109
Klíčová slova: tetrabromobisphenol A * protamimation * DNA integrity * TUNEL assay * qPCR
Kód oboru RIV: EC - Imunologie

Tetrabromobisphenol A (TBBPA) is commonly used as flame retardant and currently is among the most widely used; its consumption is around 210,000 tons/year and is still growing. TBBPA has negative effect on different mammalian cell lines in vitro and it is able to affect the thyroid hormone system as well it might be able to influence the androgen/estrogen hormone system in vivo. Thus TBBPA is considered as endocrine disruptor and its possible negative effect on human and wildlife health needs to be investigated. In our previous study we have observed that TBBPA is able to induce apoptosis of testicular cells and changes in the expression of genes playing important roles during spermatogenesis. Moreover, we observed that certain effects can be transmitted to the second generation. In this study we examined in more detail the effect of low concentration of TBBPA (35μg/kg b.w.) on reproductive parameters of male mice in vivo. C57Bl/6J mouse pups were exposed to TBBPA (experimental group) during the gestation, lactation, pre-pubertal and pubertal periods up to the age of 70 days and compared to unexposed control group. We mainly focused on the analysis of protamines, the most abundant sperm nuclear proteins, and on the sperm DNA integrity. We have shown that TBBPA is able to induce changes in sperm protamination, number of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) positive sperm and serum testosterone level. We detected significantly decreased protamine 1/protamine 2 ratio (0.362 vs. 0.494; p < 0.001), increased total protamine/DNA ratio (0.517 vs. 0.324; p < 0.001) and increased number of TUNEL positive spermatozoa (39.5% vs. 21.2%; p < 0.05). Protamines recently have been implicated in the epigenetic marking of sperm chromatin in human and mouse spermatozoa. Thus, changes in sperm protamination induced by TBBPA, may explain the previously observed second generation effect.
Trvalý link: http://hdl.handle.net/11104/0262860