Study of sperm proteins in different mammalian species

0463007 - BTÚ 2017 eng K - Konferenční příspěvek (tuzemská konf.)
Pohlová, Alžběta - Zigo, Michal - Jonáková, Věra - Postlerová, Pavla
Study of sperm proteins in different mammalian species.
Book of abstracts of XXIInd Symposium of biology and immunology of reproduction. Vestec u Prahy: Biotechnologický ústav AVČR, 2016 - (Pěknicová, J.), s. 29-29
[XXIInd Symposium of biology and immunology of reproduction with international participation. Třešť (CZ), 26.05.2016-28.05.2016]
Grant CEP: GA ČR(CZ) GA 14-05547S
Institucionální podpora: RVO:86652036
Klíčová slova: sperm surface proteins * glycoproteins profiles * sperm - zona pellucida interaction * monoclonal antibodies
Kód oboru RIV: CE - Biochemie

Reproduction is an essential feature of all animals and a fundamental step to produce new generations. Study of sperm proteins is crucial for understanding of the sperm-egg recognition. We searched out sperm surface proteins involving in the zona pellucida (ZP) binding and studied whether these proteins are preserved throughout mammalian species. Indirect immunofluorescent technique was used to test a panel of monoclonal antibodies prepared against boar sperm surface proteins on spermatozoa of bull and mice. We found a cross-reactivity of some antibodies against boar sperm with bull ejaculated and mouse epididymal spermatozoa. Further, we isolated sperm proteins from different mammalian species, such as pig, bull, dog, cat, mouse and human. Proteins were separated by SDS-electrophoresis and protein/glycoprotein profiles from epididymal, ejaculated and in vitro capacitated sperm were compared. The interaction of sperm with ZP was studied on electrophoretically-separated sperm surface proteins from pig and bull with biotin-labeled ZP glycoproteins using Far Western blot. Antibodies, which reacted with boar sperm surface proteins with ZP-binding activity, therefore could be potential egg-binding receptors, were used for monitoring of the sperm protein origin in reproductive fluids and tissues.
Trvalý link: http://hdl.handle.net/11104/0262927