Possible role of spermatogenic protein glyceraldehyde-3-phosphate dehydrogenase (GAPDHS) in mammalian sperm

0462995 - BTÚ 2017 CZ eng K - Konferenční příspěvek (tuzemská konf.)
Margaryan, Hasmik - Dorosh, Andriy - Čapková, Jana - Postlerová, Pavla - Philimonenko, Anatoly - Hozák, Pavel - Pěknicová, Jana
Possible role of spermatogenic protein glyceraldehyde-3-phosphate dehydrogenase (GAPDHS) in mammalian sperm.
Book of abstracts of XXIst Symposium of biology and immunology of reproduction. Praha: Biotechnologický ústav AVČR, 2015 - (Pěknicová, J.; Elzeinová, F.; Kubátová, A.), s. 31-31
[XXIst SYMPOSIUM OF BIOLOGY AND IMMUNOLOGY OF REPRODUCTION WITH INTERNATIONAL PARTICIPATION. Třešť (CZ), 14.05.2015-16.05.2015]
Grant CEP: GA ČR(CZ) GA P503/12/1834
Klíčová slova: GAPDHS * sperm glycolytic enzyme * zona pellucida glycoprotein matrix * monoclonal antibody
Kód oboru RIV: CE - Biochemie

Sperm proteins are important for the structure and function of these specific, highly differentiated cells. Certain of these proteins play a role in sperm-egg recognition during primary or secondary binding at zona pellucida glycoprotein matrix. The aim of this study was to characterize the acrosomal sperm protein recognized by a monoclonal antibody (MoAb) Hs-8, prepared in our laboratory by immunization of BALB/c mice with human ejaculated sperm, and to test the possible role of this protein in gamete interaction. MoAb Hs-8 specifically labelled a 45 kDa protein from the sperm extract in the immunoblotting test. Sequence analysis identified this Hs-8 protein as GAPDHS. In order to perform a control tests, a commercial mouse anti-GAPDHS MoAb was applied. Both antibodies showed similar staining patterns using immunofluorescence labelling, transmission electron microscopy and immunoblot analysis. Moreover, both Hs-8 and commercial anti-GAPDHS antibodies blocked the secondary sperm-zona pellucida binding. Generally, GAPDHS was considered mainly as sperm-specific glycolytic enzyme involved in energy production during spermatogenesis and sperm motility and its role in the sperm head was unknown. In this study, we confirmed the potential additional role of GAPDHS as a binding protein that is involved in the sperm-zona pellucida interaction.
Trvalý link: http://hdl.handle.net/11104/0262906