Sperm protein profiles of different mammalian species

0462994 - BTÚ 2017 CZ eng K - Konferenční příspěvek (tuzemská konf.)
Pohlová, Alžběta - Zigo, Michal - Jonáková, Věra - Postlerová, Pavla
Sperm protein profiles of different mammalian species.
Book of abstracts of XXIst Symposium of biology and immunology of reproduction. Praha: Biotechnologický ústav AVČR, 2015 - (Pěknicová, J.; Elzeinová, F.; Kubátová, A.), s. 30-30
[XXIst SYMPOSIUM OF BIOLOGY AND IMMUNOLOGY OF REPRODUCTION WITH INTERNATIONAL PARTICIPATION. Třešť (CZ), 14.05.2015-16.05.2015]
Grant CEP: GA ČR(CZ) GA P503/12/1834; GA ČR(CZ) GA 14-05547S; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:86652036
Klíčová slova: SDS-electrophoresis * sperm proteins * zona pellucida binding activity * monoclonal antibody
Kód oboru RIV: CE - Biochemie

Proteins are a substantial equipment of the spermatic cell; therefore, the characterization of sperm proteins is crucial for explanation of molecular mechanisms in the reproduction process. We isolated sperm proteins from different mammalian species - pig, bull, human, mouse, dog and cat. Extracted proteins were separated by SDS-electrophoresis and protein/glycoprotein profiles from epididymal or ejaculated sperm were compared. Additionally, we tested cross-reactivity of antibodies prepared to sperm boar proteins on spermatozoa of other mammalian species using immunofluorescent technique. Our future plan is to compare the protein profiles of sperm during their functional development (epididymal, ejaculated, capacitated) in various mammalian species and identify species-specific sperm proteins with zona pellucida binding activity.
Trvalý link: http://hdl.handle.net/11104/0264415