Novel structural aspect of the diatom thylakoid membrane: lateral segregation of photosystem I under red-enhanced illumination

0461933 - BC 2017 RIV GB eng J - Článek v odborném periodiku
Bína, David - Herbstová, Miroslava - Gardian, Zdenko - Vácha, František - Litvín, Radek
Novel structural aspect of the diatom thylakoid membrane: lateral segregation of photosystem I under red-enhanced illumination.
Scientific Reports. Roč. 6, MAY 2016 (2016), č. článku 25583. ISSN 2045-2322. E-ISSN 2045-2322
Grant CEP: GA ČR GBP501/12/G055
Institucionální podpora: RVO:60077344
Klíčová slova: Light-harvesting complexes * Supramolecular organization * Protein complexes
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 4.259, rok: 2016

Spatial segregation of photosystems in the thylakoid membrane (lateral heterogeneity) observed in plants and in the green algae is usually considered to be absent in photoautotrophs possessing secondary plastids, such as diatoms. Contrary to this assumption, here we show that thylakoid membranes in the chloroplast of a marine diatom, Phaeodactylum tricornutum, contain large areas occupied exclusively by a supercomplex of photosystem I (PSI) and its associated Lhcr antenna. These membrane areas, hundreds of nanometers in size, comprise hundreds of tightly packed PSI-antenna complexes while lacking other components of the photosynthetic electron transport chain. Analyses of the spatial distribution of the PSI-Lhcr complexes have indicated elliptical particles, each 14 x 17 nm in diameter. On larger scales, the red-enhanced illumination exerts a significant effect on the ultrastructure of chloroplasts, creating superstacks of tens of thylakoid membranes.
Trvalý link: http://hdl.handle.net/11104/0261481