Počet záznamů: 1
Uncovering the benefits of fluctuating thermal regimes on cold tolerance of drosophila flies by combined metabolomic and lipidomic approach
- 1.0461930 - BC 2017 RIV NL eng J - Článek v odborném periodiku
Colinet, H. - Renault, D. - Javal, M. - Berková, Petra - Šimek, Petr - Košťál, Vladimír
Uncovering the benefits of fluctuating thermal regimes on cold tolerance of drosophila flies by combined metabolomic and lipidomic approach.
Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. Roč. 1861, č. 11 (2016), s. 1736-1745. ISSN 1388-1981. E-ISSN 1879-2618
Grant CEP: GA ČR GA13-18509S
Institucionální podpora: RVO:60077344
Klíčová slova: cold stress * fluctuating thermal regimes * recovery
Kód oboru RIV: ED - Fyziologie
Impakt faktor: 5.547, rok: 2016
http://www.sciencedirect.com/science/article/pii/S1388198116302281
In this study, we have shown that Fluctuating temperature regimes (FTRs) strongly promoted cold tolerance of Drosophilamelanogaster adults. We then assessed whether this marked phenotypic shift was associated with detectable physiological changes, such as synthesis of cryoprotectants and/or membrane remodeling. To test these hypotheses, we conducted two different time-series Omics analyzes in adult flies submitted to CLTs vs. FTRs: metabolomics (GC/MS) and lipidomics (LC/ESI/MS) targeting membrane phospholipids. We observed increasing levels in several polyhydric alcohols, sugars and amino acids in flies under CLT. Lipidomics revealed an increased relative proportion of phosphatidylethanolamines and a shortening of fatty acyl chains in flies exposed to cold.
Trvalý link: http://hdl.handle.net/11104/0263669
Počet záznamů: 1