Ehrlichia minasensis sp nov., isolated from the tick Rhipicephalus microplus

0461846 - BC 2017 RIV GB eng J - Článek v odborném periodiku
Cabezas-Cruz, A. - Zweygarth, E. - Vancová, Marie - Broniszewska, M. - Grubhoffer, Libor - Friche Passos, L.M. - Barbosa Ribeiro, M.F. - Alberdi, P. - de la Fuente, J.
Ehrlichia minasensis sp nov., isolated from the tick Rhipicephalus microplus.
International Journal of Systematic and Evolutionary Microbiology. Roč. 66, č. 3 (2016), s. 1426-1430. ISSN 1466-5026. E-ISSN 1466-5034
GRANT EU: European Commission(XE) 278976 - ANTIGONE
Institucionální podpora: RVO:60077344
Klíčová slova: cell lines * Anaplasma * genotype * pathogen * Brazil
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.134, rok: 2016

Recently, we obtained a rickettsial isolate (Ehrlichia sp. UFMG-EV) from the hemolymph of Rhipicephalus microplus engorged tick females. On the basis of maximum likelihood phylogenetic analysis using 16S rRNA, groEL, dsb, gltA and trp36 nucleotide sequences we showed that Ehrlichia sp. UFMG-EV belongs to the α-Proteobacteria, family Anaplasmataceae, genus Ehrlichia. Ehrlichia sp. UFMG-EV is a sister taxa of E. canis with 16S rRNA, groEL, dsb, gltA and trp36 nucleotide sequence similarity of 98.3%, 97.2%, 94.7%, 94.3% and 49.1%, respectively. Ehrlichia sp. UFMG-EV has been maintained in the laboratory by continuous passage in the IDE8 tick cell line where the ultrastructure was characterized using electron microscopy and it resembles that of E. canis, E. muris and E. chaffensis, but not E. ruminantium and E. ewingii. We propose the name Ehrlichia minasensis sp. nov. for this bacterium to acknowledge the place from where it was isolate for first time, Minas Gerais, Brazil. The type strain of Ehrlichia minasensis sp. nov. is strain Ehrlichia sp. UFMG-EV (= DSM 100393, = TCB-TBB-0018).
Trvalý link: http://hdl.handle.net/11104/0267769