RGDS- and TAT-conjugated upconversion of NaYF4:Yb3+/Er3+&SiO2 nanoparticles: in vitro human epithelioid cervix carcinoma cellular uptake, imaging, and targeting

0461624 - ÚMCH 2017 RIV US eng J - Článek v odborném periodiku
Kostiv, Uliana - Kotelnikov, Ilya - Proks, Vladimír - Šlouf, Miroslav - Kučka, Jan - Engstová, Hana - Ježek, Petr - Horák, Daniel
RGDS- and TAT-conjugated upconversion of NaYF4:Yb3+/Er3+&SiO2 nanoparticles: in vitro human epithelioid cervix carcinoma cellular uptake, imaging, and targeting.
ACS Applied Materials and Interfaces. Roč. 8, č. 31 (2016), s. 20422-20431. ISSN 1944-8244. E-ISSN 1944-8252
Grant CEP: GA ČR(CZ) GA15-01897S; GA ČR(CZ) GA16-02702S
Institucionální podpora: RVO:61389013 ; RVO:67985823
Klíčová slova: upconversion, nanoparticles * RGDS peptide * TAT peptide
Kód oboru RIV: CD - Makromolekulární chemie; BO - Biofyzika (FGU-C)
Impakt faktor: 7.504, rok: 2016

Starting NaYF4:Yb3+/Er3+ nanoparticles with size tuned from 24 to 33 nm were prepared by high-temperature coprecipitation of lanthanide chlorides in high-boiling organic solvents. To enhance colloidal stability in aqueous medium, an aminosilica shell was introduced on the surface by hydrolysis and condensation of tetramethyl orthosilicate and (3-aminopropyl)trimethoxysilane using a reverse microemulsion technique; to form alkyne groups, reaction with 4-pentynoic acid followed. Finally, the cell adhesive and cell penetrating azidopentanoyl-GGGRGDSGGGY-NH2 (RGDS) and azidopentanoyl-GGGRKKRRQRRR-NH2 (TAT) peptides were conjugated to the upconversion particles via Cu(I)-catalyzed alkyne–azide cycloaddition. The concentrations of the peptides bound to the nanoparticle surfaces and amount of adsorbed residual Cu(I) catalyst were determined using an 125I-radiolabeled RGDS peptide and a 64Cu(I)-doped catalyst, respectively. Targeting and uptake of the RGDS- and TAT-conjugated NaYF4:Yb3+/Er3+&SiO2 nanoparticles by human cervix carcinoma HeLa cells were monitored by confocal microscopy. RGDS-conjugated nanoparticle probes were mainly localized on the cell plasma membrane due to specific binding of the peptide to the corresponding integrins. In contrast, the TAT-conjugated nanoparticles were able to cross the cell membrane and accumulate in the cell cytoplasm. Thus, this new peptide bioconjugation approach supported both extra- and intracellular nanoparticle uptake, enabling targeting and imaging of the specific tumor phenotypes.
Trvalý link: http://hdl.handle.net/11104/0261814