Voltammetric and capillary electrophoretic study of scavenger kinetics of methylglyoxal by antidiabetic biguanide drugs

0461541 - ÚFCH JH 2017 RIV CH eng J - Článek v odborném periodiku
Langmaier, Jan - Samec, Zdeněk - Samcová, E. - Tůma, P.
Voltammetric and capillary electrophoretic study of scavenger kinetics of methylglyoxal by antidiabetic biguanide drugs.
Journal of Electroanalytical Chemistry. Roč. 777, SEP 2016 (2016), s. 26-32. ISSN 1572-6657. E-ISSN 1873-2569
Grant CEP: GA ČR(CZ) GA15-03139S
Institucionální podpora: RVO:61388955
Klíčová slova: ion transfer voltammetry * capillary electrophoresis * biguanide
Kód oboru RIV: CG - Elektrochemie
Impakt faktor: 3.012, rok: 2016

A novel electroanalytical approach is used to get insight into scavenger kinetics and mechanism of methylglyoxal (MG) by biguanides metformin, phenformin and 1-phenylbiguanide under the physiological conditions (pH 7.41 and 37 °C). The approach is based on monitoring the time profiles of concentrations of the protonated biguanides by ion transfer voltammetry (ITV) at the polarizable room-temperature ionic liquid membrane. Effects of the reactant's concentrations and pH lead us to propose a mechanism including an acid-base reaction of biguanide, which separates the parallel reactions of the protonated and deprotonated biguanide with methylglyoxal. Reaction rate increases considerably in the sequence metformin < phenformin < 1-phenylbiguanide. Kinetic data confirm the previous finding that scavenging of methylglyoxal by metformin is a relatively slow process. Optimization of the background electrolyte enables to use capillary electrophoresis (CE) to detect simultaneously the protonated biguanide and the MG anion in the reaction mixture. CE analysis then yields the mole-to-mole ratios of the reacted biguanides to MG in the range 0.6–0.7 pointing to the simultaneous formation of a 1:1 and a 1:2 reaction products. Usefulness of ion voltammetry or amperometry for further kinetic studies of biguanides, as well as for their assays, can be envisaged.
Trvalý link: http://hdl.handle.net/11104/0261159