Imaging of Norway spruce early somatic embryos with the ESEM, Cryo-SEM and laser scanning microscope

0461452 - ÚPT 2017 RIV GB eng J - Článek v odborném periodiku
Neděla, Vilém - Hřib, Jiří - Havel, L. - Hudec, Jiří - Runštuk, Jiří
Imaging of Norway spruce early somatic embryos with the ESEM, Cryo-SEM and laser scanning microscope.
Micron. Roč. 84, May 2016 (2016), s. 67-71. ISSN 0968-4328. E-ISSN 1878-4291
Grant CEP: GA ČR(CZ) GA14-22777S
Institucionální podpora: RVO:68081731
Klíčová slova: ESEM * Cryo-SEM * bright field/dark field microscopy * extracellular matrix * Picea abies * somatic embryogenesis
Kód oboru RIV: JA - Elektronika a optoelektronika, elektrotechnika
Impakt faktor: 1.980, rok: 2016

This article describes the surface structure of Norway spruce early somatic embryos (ESEs) as a typical culture with asynchronous development. The microstructure of extracellular matrix covering ESEs were observed using the environmental scanning electron microscope as a primary tool and using the scanning electron microscope with cryo attachment and laser electron microscope as a complementary tool allowing our results to be proven independently. The fresh samples were observed in conditions of the air environment of the environmental scanning electron microscope (ESEM) with the pressure from 550 Pa to 690 Pa and the low temperature of the sample from -18 degrees C to -22 degrees C. The samples were studied using two different types of detector to allow studying either the thin surface structure or material composition. The scanning electron microscope with cryo attachment was used for imaging frozen extracellular matrix microstructure with higher resolution. The combination of both electron microscopy methods was suitable for observation of "native" plant samples, allowing correct evaluation of our results, free of error and artifacts.
Trvalý link: http://hdl.handle.net/11104/0261042