New methodology for capillary electrophoresis with ESI-MS detection: Electrophoretic focusing on inverse electromigration dispersion gradient. High-sensitivity analysis of sulfonamides in waters

0460851 - ÚIACH 2017 RIV NL eng J - Článek v odborném periodiku
Malá, Zdeňka - Gebauer, Petr - Boček, Petr
New methodology for capillary electrophoresis with ESI-MS detection: Electrophoretic focusing on inverse electromigration dispersion gradient. High-sensitivity analysis of sulfonamides in waters.
Analytica Chimica Acta. Roč. 935, SEP (2016), s. 249-257. ISSN 0003-2670. E-ISSN 1873-4324
Grant CEP: GA ČR(CZ) GA16-09135S
Institucionální podpora: RVO:68081715
Klíčová slova: electrophoretic focusing * CE-ESI-MS * capillary electrophoresis
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 4.950, rok: 2016

This article describes for the first time the combination of electrophoretic focusing on inverse electromigration dispersion (EMD) gradient, a new separation principle described in 2010, with electrosprayionization (ESI) mass spectrometric detection. The separation of analytes along the electromigrating EMD profile proceeds so that each analyte is focused and concentrated within the profile at a particular position given by its pKa and ionic mobility. The proposed methodology combines this principle with the transport of the focused zones to the capillary end by superimposed electromigration, electroosmotic flow and ESI suction, and their detection by the MS detector. The designed electrolyte system based on maleic acid and 2,6-lutidine is suitable to create an inverse EMD gradient of required properties and its components are volatile enough to be compatible with the ESI interface.
Trvalý link: http://hdl.handle.net/11104/0260827