Structural analysis and anti-obesity effect of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.)

0460383 - ÚOCHB 2017 RIV GB eng J - Článek v odborném periodiku
Choi, J. W. - Synytsya, A. - Capek, P. - Bleha, R. - Pohl, Radek - Park, Y. I.
Structural analysis and anti-obesity effect of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.).
Carbohydrate Polymers. Roč. 146, Aug 1 (2016), s. 187-196. ISSN 0144-8617. E-ISSN 1879-1344
Institucionální podpora: RVO:61388963
Klíčová slova: mulberry fruit * pectic polysaccharide * structure * pre-adipocytes * apoptosis
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 4.811, rok: 2016

A water-soluble polysaccharide JS-MP-1 was isolated from Korean mulberry fruits Oddi (Morus alba L.). Sugar linkage analysis and NMR data confirmed that it is a rhamnogalacturonan type I (RG I) polymer carrying arabinan and arabinogalactan (AG II) side chains. JS-MP-1 reduced dose-dependently the viability of 3T3-L1 pre-adipocyte cells, significantly stimulated the cleavage of caspases 9 and 3 and poly (ADP-ribose) polymerase (PARP) and decreased the ratio of Bcl-2 to Bax expression level that led to mitochondrial dysfunction and apoptosis in pre-adipocyte cells. The apoptotic death was mediated by stimulation of MAPKs (ERK and p38) signalling pathway. These results suggest that JS-MP-1 is able to reduce the number of fat cells and the mass of adipose tissue via inhibition of pre-adipocyte proliferation and thus JS-MP-1 itself or a crude aqueous Oddi extract containing this polysaccharide can be used as functional ingredient of health-beneficial food supplements for the treatment or prevention of obesity disorders.
Trvalý link: http://hdl.handle.net/11104/0260488