Manumycin A downregulates release of proinflammatory cytokines from TNF alpha stimulated human monocytes

0460046 - MBÚ 2017 RIV NL eng J - Článek v odborném periodiku
Cecrdlová, E. - Petříčková, Kateřina - Kolesár, L. - Petříček, Miroslav - Sekerková, A. - Šváchová, V. - Stříž, I.
Manumycin A downregulates release of proinflammatory cytokines from TNF alpha stimulated human monocytes.
Immunology Letters. Roč. 169, JAN 2016 (2016), s. 8-14. ISSN 0165-2478. E-ISSN 1879-0542
Grant CEP: GA MŠMT LH12191; GA MZd(CZ) NT13012
Institucionální podpora: RVO:61388971
Klíčová slova: Manumycin A * Cytokines * Immunomodulation
Kód oboru RIV: EC - Imunologie
Impakt faktor: 2.860, rok: 2016

Macrolide antibiotics such as azithromycin or clarithromycin are known to have potent anti-inflammatory and immunomodulatory effects but these properties cannot be widely used due to a risk of bacterial resistance. We studied another polyketide antibiotic, structurally related manumycin A known as a streptomycete derived farnesyltransferase inhibitor with limited antibacterial effects, with respect to its potential regulation of mRNA expression of several genes associated with proinflammatory responses. Downregulation of mRNA for IL-6, TLR-8, IL-1 beta and IL-10 was found in THP-1 cells after 4 h stimulation with TNF alpha in the presence of manumycin A and downregulated TLR-8 and EGR-1 genes were observed after 8 h. Among the genes upregulated in response to manumycin were HMOX-1, TNFRSF10A, IL-1R1, TICAM2, NLRP12 after 4 h and only IL-1R1 after 8 h.
Trvalý link: http://hdl.handle.net/11104/0260208