The effect of diabetes mellitus on reproductive parameters and expression of selected testicular genes in streptozotocin-induced diabetic mice.\n\n\n

0459814 - BTÚ 2017 CZ eng A - Abstrakt
Valášková, Eliška - Žatecká, Eva - Dorosh, Andriy - Pavlínková, Gabriela - Bohuslavová, Romana - Elzeinová, Fatima - Kubátová, Alena - Margaryan, Hasmik - Dvořáková-Hortová, Kateřina - Pěknicová, Jana
The effect of diabetes mellitus on reproductive parameters and expression of selected testicular genes in streptozotocin-induced diabetic mice.


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Book of abstract XXIInd Symposium of immunology and biology of Reproduction with international participation. Vestec u Prahy: Biotechnologický ústav, 2016 - (Pěknicová, J.; Kubátová, A.; Elzeinová, F.). s. 42-43
[XXIInd Symposium of Immunology and Biology of Reproduction with international participation. 26.05.2016-28.05.2016, Třešť]
Grant CEP: GA MŠMT(CZ) ED1.1.00/02.0109; GA MZd(CZ) AZV 15-30880A
Institucionální podpora: RVO:86652036
Klíčová slova: diabetes mellitus * testicular genes analysis * streptozotocin * annexin V * sperm parameters
Kód oboru RIV: EB - Genetika a molekulární biologie

According to the World Health Organization (WHO), 15% of couples in reproductive age suffer from infertility problems, and up to 60% of cases are caused by male factor. Causes of this condition could be genetic background, environmental factors and various diseases, including diabetes mellitus (DM). However, the impact of DM on male fertility is not fully understood. The aim of this study was to investigate the effects of DM on reproduction and expression of selected testicular genes using FVB inbred mouse strain, suitable for inducing DM and subsequent reproductive parameters analysis. This mouse strain is characterized by vigorous reproductive performance and consistently large litters.
DM (type 1) was artificially induced in the age of 6 weeks by chemical substance streptozotocin, which causes destruction of pancreatic β cells. Mice with blood sugar levels higher than 13.9 mmol/L were considered as diabetic. These mice were exposed to diabetic condition for 6 weeks and then subjected to analysis.
Our results have shown that diabetic condition had an impact on body weight, weight of reproductive organs as well as kidneys and livers. We also evaluated sperm quality by analysing several sperm parameters (morphology, viability, concentration, apoptotic status). We observed decreased concentration and viability of diabetic sperm compared to control. We also noticed increased staining with apoptotic marker annexin V. Moreover, we evaluated the qualitative and quantitative changes of sperm nuclear proteins - protamines. In diabetic animals, we observed higher number of sperm with insufficient protamination. Nevertheless, protamine 1 to protamine 2 ratio (P1/P2), a marker of male fertility, was not altered in sperm of diabetic animals compared to controls. Regarding the testicular tissue, we observed impaired morphology of seminiferous tubules and increased number of apoptotic cells in testes of diabetic animals. We also analysed expression of several important testicular genes (Vegfa, Sycp1, Sycp3, Ccna1, Meig1, Prm1, Prm2, Tnp1, Tnp2, Grth, Gja1, Wt1, Sox8, p21, p53, Bcl-2, Ar, Fshr, Star, p450scc), which are responsible for proper function of spermatocytes and spermatids.
Our findings indicate that DM affects sperm quality in terms of alterations in the morphology of testicular tissue. The metabolic disorders also caused changes in the expression of genes important during meiotic phase of spermatogenesis (Sycp1 and Sycp3) and genes important during spermiogenesis (Tnp1, Tnp1, Prm1 and Prm2). Changes in gene expression could further contribute to impaired sperm quality of diabetic animals.





Trvalý link: http://hdl.handle.net/11104/0259990