Super-resolution Stimulated Emission Depletion (STED) microscopy imaging of selected integrins in mouse acrosome intact sperm.\n\n

0459803 - BTÚ 2018 CZ eng A - Abstrakt
Frolíková, Michaela - Šebková, Nataša - Děd, Lukáš - Dvořáková-Hortová, Kateřina
Super-resolution Stimulated Emission Depletion (STED) microscopy imaging of selected integrins in mouse acrosome intact sperm.

.
Book of abstract XXIInd Symposium of immunology and biology of Reproduction with international participation. Vestec u Prahy: Biotechnologický ústav, 2016. s. 17-18.
[XXIInd Symposium of Immunology and Biology of Reproduction with international participation. 26.05.2016-28.05.2016, Třešť]
Grant CEP: GA ČR GA14-05547S; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:86652036
Klíčová slova: STED * integrin * sperm head
Obor OECD: Biophysics

The integrins are transmembrane proteins that participate in many cell-cell and cell-extracelular matrix interaction. These membrane receptors are important part of signalling pathway and they have ability to transmit signals in both directions – into and out of the cell. The integrins are able to associate with other membrane receptors in multi-molecular complexes that participate in cell activation. Integrins proteins consist of two different subunits α and β. β subunit is capable of binding to actin and controlling of its remodeling. To present, 18 α and 6 β integrin subunits and 24 their heterodimers combinations have been described in mammals. Recently, there have been certain integrins described to be expressed also on sperm. Importantly, in some cases of man infertility, there was detected reduced expression of integrins and positive correlation between expression of integrins and fertilizing ability of human spermatozoa was shown. For this reason integrins were proposed as a potential clinical marker to evaluate man sperm quality. However, up to date, there is lack of information about distribution of individual integrins heterodimers and their function on mature sperm. Therefore, we set the aim to investigate and characterize the presence of selected integrins subunits and their accurate position in acrosome intact sperm using mouse model for a start. Dual fluorescent labeling and STED super resolution microscopy was used for localization of the studied proteins. So far we have described the expression of tree subunits α3, α6 and β1 on acrosome intact mouse sperm. The presence of α3 was detected on outer acrosomal membrane and plasma membrane of acrosomal cap area. β1 subunit was present in same structures and even in plasma membrane of the apical hook. α6 was found in plasma membrane of apical hook and equatorial segment.
Trvalý link: http://hdl.handle.net/11104/0259984