Distribution of CD46 and β1 integrin molecules with respect to different membrane structures of the sperm head.\n

0459802 - BTÚ 2018 CZ eng A - Abstrakt
Šebková, Nataša - Frolíková, Michaela - Děd, Lukáš - Dvořáková-Hortová, Kateřina
Distribution of CD46 and β1 integrin molecules with respect to different membrane structures of the sperm head.
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Book of abstracts XXIInd Symposium of Immunology and Biology of Reproduction with international participation. Vestec u Prahy: Biotechnologický ústav, 2016 - (Pěknicová, J.; Kubátová, A.; Elzeinová, F.). s. 15-15
[XXIInd Symposium of Immunology and Biology of Reproduction with international participation. 26.05.2016-28.05.2016, Třešť]
Grant CEP: GA ČR GA14-05547S; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:86652036
Klíčová slova: CD46 * beta 1 integrin * sperm head * Izumo * acrosomal reaction
Obor OECD: Biochemistry and molecular biology

CD46 protein plays an important role during fertilization and its role is associated with acrosome stability. CD46 is probably involved in signalling pathways triggering the acrosome reaction (AR). It also associates, through membrane integrins, with specific MAP kinases involved in the AR.
Our aim was to monitor possible dynamics of relocation and movement of CD46 and β1 integrin during sperm maturation and its preparation for the fertilization. The possible dependence of this localization changes on the dynamic of actin cytoskeleton was studied.
Our results show changes in the localization of these proteins associated with the AR and their mutual co-localization was observed using proximity ligation kit Duolink. After the acrosome reaction, CD46 and β1integrin spreads across the sperm head, entering the post-acrosomal compartment, and permeates the borders of different domains. The quantitative analysis of protein relocation dynamics was performed using the fluorescent intensities of CD46 and β1integrin dual staining. The redistribution of fluorescent signals was showed among the individual segments of the sperm head.
It was shown previously that actin dynamics is necessary for acrosome reaction-associated translocation of Izumo1 protein that is required for sperm-egg fusion. The question rose whether CD46 and β1 integrin proteins are also affected by actin dynamic. Cytochalasin B, a toxin binding to actin filaments and blocking its polymerization, was used during sperm incubation. This toxin significantly shifted the ability of sperm to relocate both the CD46 and β1 integrin when the AR was induced by calcium ionophore. The co-incubation of capacitated sperm with Cytochalasin B lead to a dramatic decrease of the percentages of sperm, which express relocation pattern after induced AR for both CD46 (~30%) and β1 integrin (~40%) compared to the control group with relocation pattern of both proteins in 90% for sperm.

Trvalý link: http://hdl.handle.net/11104/0259983