Hybrid Sterility Locus on Chromosome X Controls Meiotic Recombination Rate in Mouse

0459797 - ÚMG 2017 RIV US eng J - Článek v odborném periodiku
Balcová, Mária - Faltusová, Barbora - Gergelits, Václav - Bhattacharyya, Tanmoy - Mihola, Ondřej - Trachtulec, Zdeněk - Knopf, Corinna - Fotopulosová, Vladana - Chvátalová, Irena - Gregorová, Soňa - Forejt, Jiří
Hybrid Sterility Locus on Chromosome X Controls Meiotic Recombination Rate in Mouse.
PLoS Genetics. Roč. 12, č. 4 (2016), e1005906-e1005906. ISSN 1553-7404. E-ISSN 1553-7404
Grant CEP: GA MŠMT(CZ) ED1.1.00/02.0109; GA ČR GA13-08078S; GA ČR(CZ) GA14-20728S
Institucionální podpora: RVO:68378050
Klíčová slova: Histochine H3 methyltransferase * Crossing-Over * Complex Traits
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 6.100, rok: 2016

Meiotic recombination safeguards proper segregation of homologous chromosomes into gametes, affects genetic variation within species, and contributes to meiotic chromosome recognition, pairing and synapsis. The Prdm9 gene has a dual role, it controls meiotic recombination by determining the genomic position of crossover hotspots and, in infertile hybrids of house mouse subspecies Mus m. musculus (Mmm) and Mus m. domesticus (Mmd), it further functions as the major hybrid sterility gene. In the latter role Prdm9 inter-acts with the hybrid sterility X 2 (Hstx2) genomic locus on Chromosome X (Chr X) by a still unknown mechanism. Here we investigated the meiotic recombination rate at the genomewide level and its possible relation to hybrid sterility. Using immunofluorescence microscopy we quantified the foci of MLH1 DNA mismatch repair protein, the cytological counterparts of reciprocal crossovers, in a panel of inter-subspecific chromosome substitution strains. Two autosomes, Chr 7 and Chr 11, significantly modified the meiotic recombination rate, yet the strongest modifier, designated meiotic recombination 1, Meir1, emerged in the 4.7 Mb Hstx2 genomic locus on Chr X. The male-limited transgressive effect of Meir1 on recombination rate parallels the male-limited transgressive role of Hstx2 in hybrid male sterility. Thus, both genetic factors, the Prdm9 gene and the Hstx2/Meir1 genomic locus, indicate a link between meiotic recombination and hybrid sterility. A strong female-specific modifier of meiotic recombination rate with the effect opposite to Meir1 was localized on Chr X, distally to Meir1. Mapping Meir1 to a narrow candidate interval on Chr X is an important first step towards positional cloning of the respective gene(s) responsible for variation in the global recombination rate between closely related mouse subspecies.
Trvalý link: http://hdl.handle.net/11104/0259966