Počet záznamů: 1
Phylogenetic position of Geitleribactron purpureum (Synechococcales, Cyanobacteria/Cyanophyceae) and its implications for the taxonomy of Chamaesiphonaceae and Leptolyngbyaceae.
- 1.0459752 - BC 2017 RIV CZ eng J - Článek v odborném periodiku
Mareš, Jan - Cantonati, M.
Phylogenetic position of Geitleribactron purpureum (Synechococcales, Cyanobacteria/Cyanophyceae) and its implications for the taxonomy of Chamaesiphonaceae and Leptolyngbyaceae.
Fottea. Roč. 16, č. 1 (2016), s. 104-111. ISSN 1802-5439. E-ISSN 1805-4927
Institucionální podpora: RVO:60077344
Klíčová slova: Alps * carbonate lakes * Geitleribactron * heteropolar cyanobacteria * single-colony sequencing * unicellular cyanobacteria
Kód oboru RIV: EF - Botanika
Impakt faktor: 1.350, rok: 2016
Over the last decades, the taxonomy of cyanobacteria has been considerably improved and restructured due to the increase in data output from molecular phylogeny. Recently, a new protocol was developed that enables reliable sequencing of 16S rRNA genes in cultivation-resistant cyanobacteria using analysis of single cells, filaments, or colonies. In the current study, we examined a sample of a heteropolar unicellular cyanobacterium, Geitleribactron purpureum, from the holotype material (deep epilithon of Lake Tovel, Western Dolomites, Italy). We isolated and purified single colonies of G. purpureum, and subjected them to direct PCR and 16S rRNA gene sequencing. We obtained a congruent set of sequences that formed a unique, isolated cyanobacterial lineage, showing phylogenetic clustering among simple filamentous genera of the family Leptolyngbyaceae. We provide evidence for deep polyphyly in Chamaesiphonaceae, and suggest that Geitleribactron should be re-classified in the Leptolyngbyaceae.
Trvalý link: http://hdl.handle.net/11104/0259909
Počet záznamů: 1