Metabolomic profiling of urinary changes in mice with monosodium glutamate-induced obesity

0459565 - MBÚ 2017 RIV DE eng J - Článek v odborném periodiku
Pelantová, Helena - Bártová, Simona - Anýž, J. - Holubová, Martina - Železná, Blanka - Maletínská, Lenka - Novák, D. - Lacinová, Z. - Šulc, Miroslav - Haluzík, M. - Kuzma, Marek
Metabolomic profiling of urinary changes in mice with monosodium glutamate-induced obesity.
Analytical and Bioanalytical Chemistry. Roč. 408, č. 2 (2016), s. 567-578. ISSN 1618-2642. E-ISSN 1618-2650
Grant CEP: GA ČR GA13-14105S; GA MŠMT LO1509
Institucionální podpora: RVO:61388971 ; RVO:61388963
Klíčová slova: Mouse model * Monosodium glutamate (MSG) induced obesity * Diabetes
Kód oboru RIV: CE - Biochemie
Impakt faktor: 3.431, rok: 2016

Obesity with related complications represents a widespread health problem. The etiopathogenesis of obesity is often studied using numerous rodent models. The mouse model of monosodium glutamate (MSG)-induced obesity was exploited as a model of obesity combined with insulin resistance. The aim of this work was to characterize the metabolic status of MSG mice by NMR-based metabolomics in combination with relevant biochemical and hormonal parameters. NMR analysis of urine at 2, 6, and 9 months revealed altered metabolism of nicotinamide and polyamines, attenuated excretion of major urinary proteins, increased levels of phenylacetylglycine and allantoin, and decreased concentrations of methylamine in urine of MSG-treated mice. Altered levels of creatine, citrate, succinate, and acetate were observed at 2 months of age and approached the values of control mice with aging. The development of obesity and insulin resistance in 6-month-old MSG mice was also accompanied by decreased mRNA expressions of adiponectin, lipogenetic and lipolytic enzymes and peroxisome proliferator-activated receptor-gamma in fat while mRNA expressions of lipogenetic enzymes in the liver were enhanced. At the age of 9 months, biochemical parameters of MSG mice were normalized to the values of the controls. This fact pointed to a limited predictive value of biochemical data up to age of 6 months as NMR metabolomics confirmed altered urine metabolic composition even at 9 months
Trvalý link: http://hdl.handle.net/11104/0259747