Identification and bioactivity evaluation of the first neuropeptide from the lesser-known insect order Embioptera (webspinner)

0459064 - BC 2017 RIV AT eng J - Článek v odborném periodiku
Gäde, G. - Šimek, Petr - Marco, H. G.
Identification and bioactivity evaluation of the first neuropeptide from the lesser-known insect order Embioptera (webspinner).
Amino Acids. Roč. 48, č. 7 (2016), s. 1677-1684. ISSN 0939-4451. E-ISSN 1438-2199
Grant CEP: GA ČR GA13-18509S
Institucionální podpora: RVO:60077344
Klíčová slova: insects * webspinner * Embioptera
Kód oboru RIV: CE - Biochemie
Impakt faktor: 3.173, rok: 2016
http://link.springer.com/article/10.1007%2Fs00726-016-2229-9

A species of the poorly studied order Embioptera, the webspinner Oligotoma saundersii, was investigated for the peptides of the adipokinetic hormone (AKH) family. A methanolic extract of its corpora cardiaca (CC) effects carbohydrate mobilization in the cockroach, Periplaneta americana, and LC-ESI MS analysis revealed one decapeptide elucidated as pEVNFSPNWGG amide in the CC of O. saundersii. It is a novel member of the AKH family and its synthetic form has strong hypertrehalosemic activity in the American cockroach. An analog with the reversed order of the proline and asparagine residues, viz. N6 P7 -Olisa-AKH, had negligible activity thus, shedding
light on the requirements of the cockroach AKH receptor. The CC from an individual webspinner contains less than one pmol of Olisa-AKH. Comparison of the AKH sequences from the major orders of the Polyneoptera does not point to a close phylogenetic relationship between webspinners and stick insects.
Trvalý link: http://hdl.handle.net/11104/0259333