Turkish Scorzonera Species Extracts Attenuate Cytokine Secretion via Inhibition of NF-kappa B Activation, Showing Anti-Inflammatory Effect in Vitro

0458741 - ÚOCHB 2017 RIV CH eng J - Článek v odborném periodiku
Acikara, O. B. - Hošek, J. - Babula, P. - Cvačka, Josef - Buděšínský, Miloš - Dračínský, Martin - Iscan, G. S. - Kadlecová, D. - Ballová, L. - Šmejkal, K.
Turkish Scorzonera Species Extracts Attenuate Cytokine Secretion via Inhibition of NF-kappa B Activation, Showing Anti-Inflammatory Effect in Vitro.
Molecules. Roč. 21, č. 1 (2016), č. článku 43. E-ISSN 1420-3049
Institucionální podpora: RVO:61388963
Klíčová slova: anti-inflammatory activity * IL-1 beta * NF-kappa B * Scorzonera * phenolic * TNF-alpha * triterpen
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 2.861, rok: 2016
http://www.mdpi.com/1420-3049/21/1/43/htm

Scorzonera species are used in different folk medicines to combat many diseases, including the illnesses connected with inflammation. Previous experiments showed anti-inflammatory activity of Scorzonera extracts in vivo. S. latifolia, S. cana var. jacquiniana, S. tomentosa, S. mollis ssp. szowitsii, S. eriophora, S. incisa, S. cinerea, and S. parviflora extracts were, therefore, evaluated for their inhibitory activities of TNF-alpha and IL-1 beta production, and NF-kappa B nuclear translocation in THP-1 macrophages. The HPLC analysis was carried out to elucidate and to compare the composition of these extracts. Major compounds of the tested extracts have been isolated using different chromatographic techniques and further tested for their inhibitory activities on TNF-alpha and IL-1 beta production. Several extracts showed promising anti-inflammatory activity in these in vitro tests. Results of HPLC analysis revealed chlorogenic acid as a compound present in all tested extracts. Hyperoside, quercetin-3-O-beta-D-glucoside and rutin were also present in varying amount in some Scorzonera species analyzed. Furthermore, eight phenolics which were identified as quercetin-3-O-beta-D-glucoside (1), hyperoside (2), hydrangenol-8-O-glucoside (3), swertisin (4), 7-methylisoorientin (5), 4,5-O-dicaffeoyl-quinic acid (6), 3,5-di-O-caffeoyl-quinic acid (7), and chlorogenic acid (8) have been isolated as major phenolic compounds of the tested extracts and, together with eight terpenoids (9-16) previously obtained from different Scorzonera species, have been tested for the inhibition of TNF-alpha production, unfortunately with no activity comparable with standard.
Trvalý link: http://hdl.handle.net/11104/0258990