Estimation of apparent binding constant of complexes of selected acyclic nucleoside phosphonates with beta-cyclodextrin by affinity capillary electrophoresis

0458609 - ÚOCHB 2017 RIV DE eng J - Článek v odborném periodiku
Šolínová, Veronika - Mikysková, Hana - Kaiser, Martin Maxmilian - Janeba, Zlatko - Holý, Antonín - Kašička, Václav
Estimation of apparent binding constant of complexes of selected acyclic nucleoside phosphonates with beta-cyclodextrin by affinity capillary electrophoresis.
Electrophoresis. Roč. 37, č. 2 (2016), s. 239-247. ISSN 0173-0835. E-ISSN 1522-2683
Grant CEP: GA ČR(CZ) GA13-17224S; GA ČR(CZ) GA15-01948S
Institucionální podpora: RVO:61388963
Klíčová slova: acyclic nucleoside phosphonates * affinity capillary electrophoresis * binding constant * nucleotide analogs * beta-cyclodextrin
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 2.744, rok: 2016

Affinity capillary electrophoresis (ACE) has been applied to estimation of apparent binding constant of complexes of (R,S)-enantiomers of selected acyclic nucleoside phosphonates (ANPs) with chiral selector beta-cyclodextrin (beta CD) in aqueous alkaline medium. The non-covalent interactions of five pairs of (R,S)-enantiomers of ANPs-based antiviral drugs and their derivatives with beta CD were investigated in the background electrolyte (BGE) composed of 35 or 50 mM sodium tetraborate, pH 10.0, and containing variable concentration (0-25 mM) of beta CD. The apparent binding constants of the complexes of (R,S)-enantiomers of ANPs with CD were estimated from the dependence of effective electrophoretic mobilities of (R,S)-enantiomers of ANPs (measured simultaneously by ACE at constant reference temperature 25 degrees C inside the capillary) on the concentration of beta CD in the BGE using different nonlinear and linear calculation methodologies. Nonlinear regression analysis provided more precise and accurate values of the binding constants and a higher correlation coefficient as compared to the regression analysis of the three linearized plots of the effective mobility dependence on beta CD concentration in the BGE. The complexes of (R,S)-enantiomers of ANPs with beta CD have been found to be relatively weak - their apparent binding constants determined by the nonlinear regression analysis were in the range 13.3-46.4 L/mol whereas the values from the linearized plots spanned the interval 12.3-55.2 L/mol.
Trvalý link: http://hdl.handle.net/11104/0258855