Recent developments in capillary and microchip electroseparations of peptides (2013-middle 2015)

0458541 - ÚOCHB 2017 RIV DE eng J - Článek v odborném periodiku
Kašička, Václav
Recent developments in capillary and microchip electroseparations of peptides (2013-middle 2015).
Electrophoresis. Roč. 37, č. 1 (2016), s. 162-188. ISSN 0173-0835. E-ISSN 1522-2683
Grant CEP: GA ČR(CZ) GA13-17224S; GA ČR(CZ) GA15-01948S
Institucionální podpora: RVO:61388963
Klíčová slova: capillary electrochromatography * capillary electrophoresis * peptides * proteins
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 2.744, rok: 2016

The review brings a comprehensive survey of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, micropreparation, purification, and physicochemical and biochemical characterization of peptides in the years 2013, 2014, and ca. up to the middle of 2015. Advances in the investigation of electromigration properties of peptides, in the methodology of their analysis, including sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, as well as in detection of peptides, are described. New developments in particular CE and CEC modes are presented and several types of their applications to peptide analysis are reported: conventional qualitative and quantitative analysis, determination in complex (bio) matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence, and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC techniques to provide important physicochemical characteristics of peptides are demonstrated.
Trvalý link: http://hdl.handle.net/11104/0258803