Cell type-specific modulation of lipid mediator's formation in murine adipose tissue by omega-3 fatty acids

0458422 - FGÚ 2017 RIV US eng J - Článek v odborném periodiku
Kuda, Ondřej - Rombaldová, Martina - Janovská, Petra - Flachs, Pavel - Kopecký, Jan
Cell type-specific modulation of lipid mediator's formation in murine adipose tissue by omega-3 fatty acids.
Biochemical and Biophysical Research Communications. Roč. 469, č. 3 (2016), s. 731-736. ISSN 0006-291X. E-ISSN 1090-2104
Grant CEP: GA ČR(CZ) GP13-04449P; GA ČR(CZ) GA13-00871S; GA MŠMT(CZ) LH14040
Institucionální podpora: RVO:67985823
Klíčová slova: adipose tissue macrophages * omega-3 PUFA * protectin D1 * lipid mediators * lipidomics
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.466, rok: 2016

The fractionation and metabolipidomic analysis were used to identify cells producing lipid mediators in epididymal WAT of mice fed for 5 weeks obesogenic high-fat diet (lipid content 35% wt/wt), which was supplemented or not by omega-3 PUFA (4.3 mg eicosapentaenoic acid and 14.7 mg docosahexaenoic acid per g of diet). Our results demonstrate selective increase in levels of anti-inflammatory lipid mediators in WAT in response to omega-3, reflecting either their association with adipocytes (endocannabinoid-related N-docosahexaenoylethanolamine) or with stromal vascular cells (pro-resolving lipid mediator protectin D1). These results further document specific roles of various cell types in WAT in control of WAT inflammation and metabolism and they suggest that also other cells but ATMs are engaged in production of proresolving lipid mediators in response to omega-3 PUFA.
Trvalý link: http://hdl.handle.net/11104/0258694