Erv14 cargo receptor participates in yeast salt tolerance via its interaction with the plasma-membrane Nha1 cation/proton antiporter

0458420 - FGÚ 2017 RIV NL eng J - Článek v odborném periodiku
Rosas-Santiago, P. - Zimmermannová, Olga - Vera-Estrella, R. - Sychrová, Hana - Pantoja, O.
Erv14 cargo receptor participates in yeast salt tolerance via its interaction with the plasma-membrane Nha1 cation/proton antiporter.
Biochimica Et Biophysica Acta-Biomembranes. Roč. 1858, č. 1 (2016), s. 67-74. ISSN 0005-2736. E-ISSN 1879-2642
Institucionální podpora: RVO:67985823
Klíčová slova: Erv14p * Nha1p * protein–protein interaction * mislocalization * salt-tolerance * yeast
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 3.498, rok: 2016

The yeast Nha1p Na+, K+/H+ antiporter has a house-keeping role in pH and cation homeostasis. It is also needed to alleviate excess Na+ or K+ from the cytoplasm under high external concentrations of these cations. Erv14p, a putative cargo receptor for transmembrane proteins is required for trafficking of Nha1p from the endoplasmic reticulum to the plasma membrane. Sensitivity to high Na+ concentrations of the erv14 mutant associated to the intracellular mislocalization of Nha1p-GFP, together with a lower Na+ efflux, indicate the involvement of this mutual association to accomplish the survival of the yeast cell upon sodium stress. This observation is supported by the protein-protein interaction between Erv14p and Nha1p detected by the mating-based Split Ubiquitin System and co-immunoprecipitation assays. Our results indicate that even though Erv14p interacts with Nha1p through the TMD, the C-terminal is important not only for the efficient delivery of Nha1p to the plasma membrane but also for its dimerization to accomplish its role in yeast salt tolerance.
Trvalý link: http://hdl.handle.net/11104/0258692