Development of gut inflammation in mice colonized with mucosa-associated bacteria from patients with ulcerative colitis

0456525 - MBÚ 2016 RIV GB eng J - Článek v odborném periodiku
Du, Zhengyu - Hudcovic, Tomáš - Mrázek, Jakub - Kozáková, Hana - Šrůtková, Dagmar - Schwarzer, Martin - Tlaskalová, Helena - Kostovčík, Martin - Kverka, Miloslav
Development of gut inflammation in mice colonized with mucosa-associated bacteria from patients with ulcerative colitis.
Gut Pathogens. Roč. 7, č. 32 (2015). ISSN 1757-4749. E-ISSN 1757-4749
Grant CEP: GA ČR(CZ) GAP304/11/1252; GA ČR GAP303/12/0535; GA MŠMT(CZ) ED1.1.00/02.0109; GA MZd(CZ) NV15-28064A
Institucionální podpora: RVO:61388971 ; RVO:67985904
Klíčová slova: Dysbiosis * Microbiota * Germ-free mice
Kód oboru RIV: EC - Imunologie
Impakt faktor: 2.816, rok: 2015

None of the HMA mice developed colitis spontaneously. When treated with DSS, mice in F4 generation of one line of colonized mice (aHMA) developed colitis. Compared to the DSS-resistant earlier generations of aHMA mice, the F4 generation have increased abundance of Clostridium difficile and decrease abundance of C. symbiosum in their cecum contents measured by denaturing gradient gel electrophoresis and DNA sequencing. In our study, mucosa-associated microbes of UC patients were not able to induce spontaneous colitis in gnotobiotic BALB/c mice but they were able to increase the susceptibility to DSS-induced colitis, once the potentially deleterious microbes found a suitable niche.
Trvalý link: http://hdl.handle.net/11104/0257506