Voltammetric Behavior of 4-Aminophthalimide Label using Hanging Mercury Drop Electrode

0456524 - ÚOCHB 2016 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Ferenčíková, Z. - Daňhel, Aleš - Riedl, Jan - Hocek, Michal - Fojta, Miroslav
Voltammetric Behavior of 4-Aminophthalimide Label using Hanging Mercury Drop Electrode.
Sborník přednášek mezinárodní odborné konference XXXIV. Moderní Elektrochemické Metody. Ústí nad Labem: Best Servis, 2014 - (Navrátil, T.; Fojta, M.; Pecková, K.), s. 36-39. ISBN 978-80-905221-2-1.
[Moderní Elektrochemické Metody /34./. Jetřichovice (CZ), 19.05.2014-23.05.2014]
Grant CEP: GA ČR GBP206/12/G151
Institucionální podpora: RVO:68081707 ; RVO:61388963
Klíčová slova: labeled nucleotide * 4-aminophthalimide * primer extension * DNA * hanging mercury drop electrode * voltammetry
Kód oboru RIV: CG - Elektrochemie

4-aminophthalimide (API), covalently attached to nucleotide (dCTP or dATP) has recently beendesigned as a fluorescent label for the detection of DNA-protein interaction. However API is electrochemically reducible and therefore it is possible to use alternatively the API-modified nuclotides as DNA redox labels. The electrochemical behavior of labeled nucleotides and labeled DNA was studied using cyclic voltammetry and transfer stripping cyclic voltammetry at hanging mercury drop electrode. Observed CVs showed irreversible reduction signals of 4-aminophthalimide label without production of consequently oxidizable/reducible species.
Trvalý link: http://hdl.handle.net/11104/0257029