Počet záznamů: 1  

Multilocus Sequence Typing of Cronobacter Strains Isolated from Retail Foods and Environmental Samples

  1. 1.
    0456506 - ÚŽFG 2016 RIV US eng J - Článek v odborném periodiku
    Killer, Jiří - Skřivanová, E. - Hochel, I. - Marounek, M.
    Multilocus Sequence Typing of Cronobacter Strains Isolated from Retail Foods and Environmental Samples.
    Foodborne pathogens and disease. Roč. 12, č. 6 (2015), s. 514-521. ISSN 1535-3141. E-ISSN 1556-7125
    Institucionální podpora: RVO:67985904
    Klíčová slova: Cronobacter spp.
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 2.270, rok: 2015

    Cronobacter spp. are bacterial pathogens that affect children and immunocompromised adults. In this study, we used multilocus sequence typing (MLST) to determine sequence types (STs) in 11 Cronobacter spp. strains isolated from retail foods, 29 strains from dust samples obtained from vacuum cleaners, and 4 clinical isolates. Using biochemical tests, species-specific polymerase chain reaction, and MLST analysis, 36 strains were identified as Cronobacter sakazakii, and 6 were identified as Cronobacter malonaticus. In addition, one strain that originated from retail food and one from a dust sample from a vacuum cleaner were identified on the basis of MLST analysis as Cronobacter dublinensis and Cronobacter turicensis, respectively. Cronobacter spp. strains isolated from the retail foods were assigned to eight different MLST sequence types, seven of which were newly identified. The strains isolated from the dust samples were assigned to 7 known STs and 14 unknown STs. Three clinical isolates and one household dust isolate were assigned to ST4, which is the predominant ST associated with neonatal meningitis. One clinical isolate was classified based on MLST analysis as Cronobacter malonaticus and belonged to an as-yet-unknown ST. Three strains isolated from the household dust samples were assigned to ST1, which is another clinically significant ST. It can be concluded that Cronobacter spp. strains of different origin are genetically quite variable. The recovery of C. sakazakii strains belonging to ST1 and ST4 from the dust samples suggests the possibility that contamination could occur during food preparation. All of the novel STs and alleles for C. sakazakii, C. malonaticus, C. dublinensis, and C. turicensis determined in this study were deposited in the Cronobacter MLST database available online (http://pubmlst.org/cronobacter/).
    Trvalý link: http://hdl.handle.net/11104/0257017

     
     
Počet záznamů: 1  

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