Comparative growth characterization of frequently used substrains of the model cyanobacterium Synechocystis sp. PCC 6803 under varying culture conditions

0456348 - ÚVGZ 2016 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Zavřel, Tomáš - Očenášová, Petra - Sinětova, M. A. - Červený, Jan
Comparative growth characterization of frequently used substrains of the model cyanobacterium Synechocystis sp. PCC 6803 under varying culture conditions.
Global Change: A Complex Challenge : Conference Proceedings. Brno: Global Change Research Centre, The Czech Academy of Sciences, v. v. i., 2015 - (Urban, O.; Šprtová, M.; Klem, K.), s. 154-157. ISBN 978-80-87902-10-3.
[Global Change: A Complex Challenge /4th/. Brno (CZ), 23.03.2015-24.03.2015]
Grant CEP: GA MŠMT(CZ) LO1415; GA MŠMT(CZ) ED1.1.00/02.0073; GA MŠMT(CZ) EE2.3.20.0256
Institucionální podpora: RVO:67179843
Klíčová slova: growth characterization * cyanobacterium Synechocystis * culture conditions
Kód oboru RIV: EH - Ekologie - společenstva

Cyanobacteria have gained increased attention as ideal candidates for biotechnological applications due to their capacity to produce valuable molecules ranging from therapeutic proteins to biofuels. Their natural phenotypic plasticity in highly dynamic environments enables easy deployment of new biotechnologies as well as opening possibilities for genetic engineering. This contribution presents a new approach to fast and reliable characterization of cyanobacteria growth in a flat panel photobioreactor that enables examination of changing light, temperature, and nutrient availability. The utilization of semi-continuous automatic cultivation with real-time culture growth monitoring provides a strong experimental basis for both characterization and optimization of cyanobacteria cultures in photobioreactors. We first characterized the autotrophic growth of the substrain of Synechocystis sp. PCC 6803 denoted as GT-L. This strain is capable of efficient growth under a wide range of environmental conditions with doubling time as fast as 5 h under favorable conditions. However, differences among Synechocystis sp. PCC 6803 substrains have been identified on both the genotype and phenotype levels. We therefore aimed to utilize the experimental platform to characterize multiple commonly used Synechocystis sp. PCC 6803 substrains. This method will enable us to identify substrains capable of robust growth and high production yields as reliable biotechnological candidates.
Trvalý link: http://hdl.handle.net/11104/0256879