Interactions of fluorescent dye SYBR Green I with natural and 7-deazaguanine-modified DNA studied by fluorescence and electrochemical methods

0456297 - BFÚ 2016 RIV AT eng J - Článek v odborném periodiku
Dudová, Zdenka - Špaček, Jan - Havran, Luděk - Pivoňková, Hana - Fojta, Miroslav
Interactions of fluorescent dye SYBR Green I with natural and 7-deazaguanine-modified DNA studied by fluorescence and electrochemical methods.
Monatshefte fur Chemie. Roč. 147, č. 1 (2016), s. 13-20. ISSN 0026-9247. E-ISSN 1434-4475
Institucionální podpora: RVO:68081707
Klíčová slova: 7-Deazaguanine * PCR * Fluorescence
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 1.282, rok: 2016

YBR Green I (SG) is a fluorescent dye applied in various techniques of DNA analysis, including fluorescent staining of electrophoretic gels, quantitative polymerase chain reaction, etc. SG binds selectively to double-stranded DNA via intercalation and minor groove interactions, resulting in a considerable enhancement of fluorescence of the dye. Modification of DNA by partial or full replacement of natural purine nucleobase guanine (G) with its synthetic analog 7-deazaguanine (G*) or its derivatives was shown to cause the SG fluorescence quenching. In this paper, we present a comparative study of interactions of SG with natural DNA fragments and with DNA fragments modified with G* by means of fluorescence and electrochemical methods. Competition between unmodified (forming strongly fluorescent complex with SG) and fully G*-modified (not contributing significantly to overall fluorescence signal) DNA fragments for the dye was studied via changes in the fluorescence intensity.
Trvalý link: http://hdl.handle.net/11104/0256844