Time-resolved study of microorganisms by Raman spectroscopy

0456192 - ÚPT 2016 RIV US eng C - Konferenční příspěvek (zahraniční konf.)
Samek, Ota - Hároniková, A. - Obruča, S. - Bernatová, Silvie - Ježek, Jan - Šiler, Martin - Mlynariková, K. - Zemánek, Pavel
Time-resolved study of microorganisms by Raman spectroscopy.
Clinical and Biomedical Spectroscopy and Imaging IV (Proceedings of SPIE 9537). Bellingham: SPIE, 2015, 953703:1-5. ISBN 9781628417029. ISSN 1605-7422.
[Clinical and Biomedical Spectroscopy and Imaging /4./. Munich (DE), 22.06.2015-24.06.2015]
Grant CEP: GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01; GA ČR(CZ) GA15-20645S
Institucionální podpora: RVO:68081731
Klíčová slova: raman spectroscopy * time-resolved study * red yeast * Cupriavidus necator H16
Kód oboru RIV: BH - Optika, masery a lasery

The main goal of our investigations is to focus on the basic physiological mechanisms of microorganisms (yeast and bacteria), exposed to different conditions, by time-resolved Raman spectroscopy. This study provides an insight into the mechanism of targeted stress factors or the influence of different cultivation times on species metabolism in vivo, in realtime and label free. We also focused on time-course study of physico-chemical properties of bacterial cells and cell cytoplasm with respect to the intracellular content of polyhydroxyalkanoates and to the production of yeast lipids or carotenoids.
Trvalý link: http://hdl.handle.net/11104/0256780