The actin family protein ARP6 contributes to the structure and the function of the nucleolus

0455821 - ÚMG 2016 RIV US eng J - Článek v odborném periodiku
Kitamura, H. - Matsumori, H. - Kalendová, Alžběta - Hozák, Pavel - Goldberg, I.G. - Nakao, M. - Saitoh, N. - Harata, M.
The actin family protein ARP6 contributes to the structure and the function of the nucleolus.
Biochemical and Biophysical Research Communications. Roč. 464, č. 2 (2015), s. 554-560. ISSN 0006-291X. E-ISSN 1090-2104
Grant CEP: GA MŠMT EE2.3.30.0050; GA MŠMT(CZ) ED1.1.00/02.0109
Grant ostatní: Human Frontier in Science programe(FR) RGP0017/2013
Institucionální podpora: RVO:68378050
Klíčová slova: Actin-related protein * ARP6 * Histone H2A.Z * Nucleolus * Wndchrm
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.371, rok: 2015

The actin family members, consisting of actin and actin-related proteins (ARPs), are essential components of chromatin remodeling complexes. ARP6, one of the nuclear ARPs, is part of the Snf-2-related CREB-binding protein activator protein (SRCAP) chromatin remodeling complex, which promotes the deposition of the histone variant H2A.Z into the chromatin. In this study, we showed that ARP6 influences the structure and the function of the nucleolus. ARP6 is localized in the central region of the nucleolus, and its knockdown induced a morphological change in the nucleolus. We also found that in the presence of high concentrations of glucose ARP6 contributed to the maintenance of active ribosomal DNA (rDNA) transcription by placing H2A.Z into the chromatin. In contrast, under starvation, ARP6 was required for cell survival through the repression of rDNA transcription independently of H2A.Z. These findings reveal novel pleiotropic roles for the actin family in nuclear organization and metabolic homeostasis. (C) 2015 Elsevier Inc. All rights reserved.
Trvalý link: http://hdl.handle.net/11104/0256441