Possible function of glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein GAPDHS in mammalian sperm

0454481 - BTÚ 2016 US eng A - Abstrakt
Margaryan, Hasmik - Dorosh, Andriy - Čapková, Jana - Maňásková-Postlerová, Pavla - Philimonenko, Anatoly - Hozák, Pavel - Pěknicová, Jana
Possible function of glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein GAPDHS in mammalian sperm.
Abstracts of the 14th International Symposium for Immunology of Reproduction “Progress in Reproductive Immunology”. Hoboken: American Journal of Reproductive Immunology, 2015 - (Mor, G.). s. 40. ISSN 1046-7408. E-ISSN 1600-0897.
[14th International Symposium for Immunology of Reproduction "progress in Reproductive Immunology". 22.05.2015-24.05.2015, Varna]
Grant CEP: GA ČR(CZ) GAP503/12/1834; GA MŠMT(CZ) ED1.1.00/02.0109
Výzkumný záměr: CEZ:AV0Z50520701
Institucionální podpora: RVO:86652036
Klíčová slova: monoclonal antibodies * spermatozoa * GAPDHS * immunolabeling * Transmission electron microscopy * in vitro sperm/zona pellucida binding assay
Kód oboru RIV: CE - Biochemie

Sperm proteins are important in fertilization. Some of them are involved in the binding of sperm to the egg.We characterized the sperm protein detected by a monoclonal antibody (MoAb) (Hs-8) that was prepared in our laboratory. In immunoblotting test, MoAb Hs-8 labelled a protein of 45 kDa in the extract of sperm. Sequence analysis identified protein Hs-8 as GAPDHS. For this reason, commercial mouse anti-GAPDHS MoAb was applied in control tests. Both antibodies showed similar staining patterns in immunofluorescence tests, in electron microscopy and in immunoblot analysis. Moreover, both Hs-8 and anti-GAPDHS antibodies blocked sperm/zona pellucida binding. GAPDHS is a sperm-specific glycolytic enzyme involved in energy production during spermatogenesis and sperm motility; its role in the sperm head is unknown. In this study, we confirmed the potential role of GAPDHS as a binding protein that is involved in the sperm/oocyte binding.
Trvalý link: http://hdl.handle.net/11104/0255697