A combined MR and CT study for precise quantitative analysis of the avian brain

0454204 - FGÚ 2016 RIV GB eng J - Článek v odborném periodiku
Jirák, D. - Janáček, Jiří - Kear, B. P.
A combined MR and CT study for precise quantitative analysis of the avian brain.
Scientific Reports. Roč. 5, Oct 30 (2015), s. 16002. ISSN 2045-2322. E-ISSN 2045-2322
Grant CEP: GA ČR(CZ) GAP302/12/1207
Institucionální podpora: RVO:67985823
Klíčová slova: avian brain * magnetic resonance imaging * computed tomography * Fakir probe
Kód oboru RIV: EA - Morfologické obory a cytologie
Impakt faktor: 5.228, rok: 2015

Brain size is widely used as a measure of behavioural complexity and sensory-locomotive capacity in avians but has largely relied upon laborious dissections, endoneurocranial tissue displacement, and physical measurement to derive comparative volumes. As an alternative, we present a new precise calculation method based upon coupled magnetic resonance (MR) imaging and computed tomography (CT). Our approach utilizes a novel interactive Fakir probe cross-referenced with an automated CT protocol to efficiently generate total volumes and surface areas of the brain tissue and endoneurocranial space, as well as the discrete cephalic compartments. We also complemented our procedures by using sodium polytungstate (SPT) as a contrast agent. This greatly enhanced CT applications but did not degrade MR quality and is therefore practical for virtual brain tissue reconstructions employing multiple imaging modalities. To demonstrate our technique, we visualized sex-based brain size differentiation in a sample set of Ring-necked pheasants (Phasianus colchicus). This revealed no significant variance in relative volume or surface areas of the primary brain regions. Rather, a trend towards isometric enlargement of the total brain and endoneurocranial space was evidenced in males versus females, thus advocating a non-differential sexually dimorphic pattern of brain size increase amongst these facultatively flying birds
Trvalý link: http://hdl.handle.net/11104/0254907