Počet záznamů: 1
Azidopropylvinylsulfonamide as a New Bifunctional Click Reagent for Bioorthogonal Conjugations: Application for DNA-Protein Cross-Linking
- 1.0453444 - ÚOCHB 2016 RIV DE eng J - Článek v odborném periodiku
Daďová, Jitka - Vrábel, Milan - Adámik, Matěj - Brázdová, Marie - Pohl, Radek - Fojta, Miroslav - Hocek, Michal
Azidopropylvinylsulfonamide as a New Bifunctional Click Reagent for Bioorthogonal Conjugations: Application for DNA-Protein Cross-Linking.
Chemistry - A European Journal. Roč. 21, č. 45 (2015), s. 16091-16102. ISSN 0947-6539. E-ISSN 1521-3765
Grant CEP: GA ČR GBP206/12/G151
Institucionální podpora: RVO:61388963 ; RVO:68081707
Klíčová slova: biotransformations * click chemistry * conjugation * nucleic acids * proteins
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 5.771, rok: 2015
N-(3-Azidopropyl)vinylsulfonamide was developed as a new bifunctional bioconjugation reagent suitable for the cross-linking of biomolecules through copper(I)-catalyzed azide-alkyne cycloaddition and thiol Michael addition reactions under biorthogonal conditions. The reagent is easily clicked to an acetylene-containing DNA or protein and then reacts with cysteine-containing peptides or proteins to form covalent cross-links. Several examples of bioconjugations of ethynyl- or octadiynyl-modified DNA with peptides, p53 protein, or alkyne-modified human carbonic anhydrase with peptides are given.
Trvalý link: http://hdl.handle.net/11104/0254254
Počet záznamů: 1