Počet záznamů: 1
Bordetella adenylate cyclase toxin: a unique combination of a pore-forming moiety with a cell-invading adenylate cyclase enzyme
- 1.0451704 - MBÚ 2016 RIV GB eng J - Článek v odborném periodiku
Mašín, Jiří - Osička, Radim - Bumba, Ladislav - Šebo, Peter
Bordetella adenylate cyclase toxin: a unique combination of a pore-forming moiety with a cell-invading adenylate cyclase enzyme.
Pathogens and Disease. Roč. 73, č. 8 (2015). ISSN 2049-632X. E-ISSN 2049-632X
Grant CEP: GA ČR GAP302/12/0460; GA ČR GA15-09157S; GA ČR(CZ) GA15-11851S
Institucionální podpora: RVO:61388971
Klíčová slova: adenylate cyclase toxin * membrane penetration * pore-formation
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 2.483, rok: 2015
The adenylate cyclase toxin-hemolysin (CyaA, ACT or AC-Hly) is a key virulence factor of the whooping cough agent Bordetella pertussis. CyaA targets myeloid phagocytes expressing the complement receptor 3 (CR3, known as alpha(M)beta(2) integrin CD11b/CD18 or Mac-1) and translocates by a poorly understood mechanism directly across the cytoplasmic membrane into cell cytosol of phagocytes an adenylyl cyclase(AC) enzyme. This binds intracellular calmodulin and catalyzes unregulated conversion of cytosolic ATP into cAMP. Among other effects, this yields activation of the tyrosine phosphatase SHP-1, BimEL accumulation and phagocyte apoptosis induction. In parallel, CyaA acts as a cytolysin that forms cation-selective pores in target membranes. Direct penetration of CyaA into the cytosol of professional antigen-presenting cells allows the use of an enzymatically inactive CyaA toxoid as a tool for delivery of passenger antigens into the cytosolic pathway of processing and MHC class I-restricted presentation, which can be exploited for induction of antigen-specific CD8(+) cytotoxic T-lymphocyte immune responses.
Trvalý link: http://hdl.handle.net/11104/0252824
Název souboru Staženo Velikost Komentář Verze Přístup Mašín_2015_FEMSJour.pdf 3 345.6 KB Vydavatelský postprint vyžádat
Počet záznamů: 1