Biologically Active Metabolites Produced by the Basidiomycete Quambalaria cyanescens

0451309 - MBÚ 2016 RIV US eng J - Článek v odborném periodiku
Stodůlková, Eva - Císařová, I. - Kolařík, Miroslav - Chudíčková, Milada - Novák, Petr - Man, Petr - Kuzma, Marek - Pavlů, B. - Černý, J. - Flieger, Miroslav
Biologically Active Metabolites Produced by the Basidiomycete Quambalaria cyanescens.
PLoS ONE. Roč. 10, č. 2 (2015). ISSN 1932-6203. E-ISSN 1932-6203
Grant CEP: GA ČR GA13-16565S; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:61388971
Klíčová slova: ENDOPHYTIC FUNGUS * SP NOV * NAPHTHOQUINONE
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 3.057, rok: 2015

Four strains of the fungus Quambalaria cyanescens (Basidiomycota: Microstromatales), were used for the determination of secondary metabolites production and their antimicrobial and biological activities. A new naphthoquinone named quambalarine A, (S)-(+)-3-(5-ethyl-tetrahydrofuran-2-yliden)-5,7,8-trihydroxy-2-oxo-1,4-naphthoquinone (1), together with two known naphthoquinones, 3-hexanoyl-2,5,7,8-tetrahydroxy-1,4-naphthoquinone (named here as quambalarine B, 2) and mompain, 2,5,7,8-tetrahydroxy-1,4-naphthoquinone (3) were isolated. Their structures were determined by single-crystal X-ray diffraction crystallography, NMR and MS spectrometry. Quambalarine A (1) had a broad antifungal and antibacterial activity and is able inhibit growth of human pathogenic fungus Aspergillus fumigatus and fungi co-occurring with Q. cyanescens in bark beetle galleries including insect pathogenic species Beauveria bassiana. Quambalarine B (2) was active against several fungi and mompain mainly against bacteria. The biological activity against human-derived cell lines was selective towards mitochondria (2 and 3); after long-term incubation with 2, mitochondria were undetectable using a mitochondrial probe. A similar effect on mitochondria was observed also for environmental competitors of Q. cyanescens from the genus Geosmithia
Trvalý link: http://hdl.handle.net/11104/0252468