Analysis of Serum Levels of Cytokines, Chemokines, Growth Factors, and Monoamine Neurotransmitters in Patients With Tick-borne Encephalitis: Identification of Novel Inflammatory Markers With Implications for Pathogenesis

0451281 - BC 2016 RIV US eng J - Článek v odborném periodiku
Palus, Martin - Formanová, P. - Salát, J. - Žampachová, E. - Elsterová, Jana - Růžek, Daniel
Analysis of Serum Levels of Cytokines, Chemokines, Growth Factors, and Monoamine Neurotransmitters in Patients With Tick-borne Encephalitis: Identification of Novel Inflammatory Markers With Implications for Pathogenesis.
Journal of Medical Virology. Roč. 87, č. 5 (2015), s. 885-892. ISSN 0146-6615. E-ISSN 1096-9071
Grant CEP: GA ČR GAP502/11/2116
Institucionální podpora: RVO:60077344
Klíčová slova: tick-borne encephalitis virus * neuroinflammation * luminex * neuroinfection
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 1.998, rok: 2015

Tick-borne encephalitis (TBE) is a leading human neuroinfection in Europe and northeastern Asia. However, the pathophysiology of TBE is not understood completely. This study sought to determine the specific serum mediators that are associated with acute TBE. The levels of 30 cytokines, chemokines, and growth factors were measured in serum samples from 87 patients with clinically and serologically confirmed acute TBE and from 32 control subjects using the Cytokine Human Magnetic 30-Plex Panel for the Luminex platform. Serum levels of the monoamine neurotransmitters serotonin, dopamine, and noradrenaline were measured via enzyme-linked immunosorbent assay. TBE virus infection elicited increased levels of the pro-inflammatory cytokines interleukin (IL)-6, IL-8, and IL-12. TBE patients had higher IL-12:IL-4 and IL-12:IL-10 ratios than control patients, reflecting the global pro-inflammatory cytokine balance. Serum levels of the monoamine neurotransmitters serotonin, dopamine, and noradrenaline were significantly lower in TBE patients than in the control group. Most interestingly, increased levels of hepatocyte growth factor and vascular endothelial growth factor were observed in TBE patients; these proteins may be novel and mechanistically important inflammatory biomarkers of TBE.
Trvalý link: http://hdl.handle.net/11104/0252488