Macroporous biodegradable cryogels of synthetic poly(.alpha.-amino acids)

0450082 - ÚMCH 2016 RIV US eng J - Článek v odborném periodiku
Sedlačík, Tomáš - Proks, Vladimír - Šlouf, Miroslav - Dušková-Smrčková, Miroslava - Studenovská, Hana - Rypáček, František
Macroporous biodegradable cryogels of synthetic poly(.alpha.-amino acids).
Biomacromolecules. Roč. 16, č. 11 (2015), s. 3455-3465. ISSN 1525-7797. E-ISSN 1526-4602
Grant CEP: GA ČR GAP108/12/1538
Institucionální podpora: RVO:61389013
Klíčová slova: cryogelation * hydrogel * scaffold
Kód oboru RIV: CD - Makromolekulární chemie
Impakt faktor: 5.583, rok: 2015

We present an investigation of the preparation of highly porous hydrogels based on biodegradable synthetic poly(α-amino acid) as potential tissue engineering scaffolds. Covalently cross-linked gels with permanent pores were formed under cryogenic conditions by free-radical copolymerization of poly[N5-(2-hydroxyethyl)-l-glutamine-stat-N5-(2-methacryloyl-oxy-ethyl)-l-glutamine] (PHEG-MA) with 2-hydrohyethyl methacrylate (HEMA) and, optionally, N-propargyl acrylamide (PrAAm) as minor comonomers. The morphology of the cryogels showed interconnected polyhedral or laminar pores. The volume content of communicating water-filled pores was >90%. The storage moduli of the swollen cryogels were in the range of 1–6 kPa, even when the water content was >95%. The enzymatic degradation of a cryogel corresponded to the decrease in its storage modulus during incubation with papain, a model enzyme with specificity analogous to wound-healing enzymes. It was shown that cryogels with incorporated alkyne groups can easily be modified with short synthetic peptides using azide-alkyne cycloaddition “click” chemistry, thus providing porous hydrogel scaffolds with biomimetic features.
Trvalý link: http://hdl.handle.net/11104/0253125