Full-length transient receptor potential vanilloid 1 channels mediate calcium signals and possibly contribute to osmoreception in vasopressin neurones in the rat supraoptic nucleus

0449670 - ÚEM 2016 RIV DE eng J - Článek v odborném periodiku
Moriya, T. - Shibasaki, R. - Kayano, T. - Takebuchi, N. - Ichimura, M. - Kitamura, N. - Asano, A. - Hosaka, Y. Z. - Forostyak, Oksana - Verkhratsky, A. - Dayanithi, Govindan - Shibuya, I.
Full-length transient receptor potential vanilloid 1 channels mediate calcium signals and possibly contribute to osmoreception in vasopressin neurones in the rat supraoptic nucleus.
Cell Calcium. Roč. 57, č. 1 (2015), s. 24-37. ISSN 0143-4160. E-ISSN 1532-1991
Grant CEP: GA ČR(CZ) GAP303/11/0192; GA ČR(CZ) GBP304/12/G069; GA ČR(CZ) GA14-34077S
Institucionální podpora: RVO:68378041
Klíčová slova: osmoregulation * oxytocin * SON * transgenic rats * TRPV1 * vasopressin
Kód oboru RIV: FH - Neurologie, neurochirurgie, neurovědy
Impakt faktor: 2.909, rok: 2015

The purpose of the present study was to uncover the structure and function of molecules related to the central osmoreceptorin the supraoptic nucleus (SON) of rats. For this purpose, we performed RT-PCR and immunohistochemistry for TRPV1-related molecules, and patch-clamp and imaging of the cytosolic Ca2+ concentration ([Ca2+]i) to measure responses to osmolality changes and TRPV-related drugs. Arginine vasopressin (AVP), synthesized in the SON is a key factor in systemic osmolality regulation. We found that AVP neurones in the SON possess functional full-length TRPV1. Moreover, differences between the responses to capsaicin or hyperosmolality obtained in rat SON neurones and those obtained from dorsal root ganglion neurones or TRPV1-expressing cells indicate that the osmoreceptor expressed in the SON may be a heteromultimer in which TRPV1 is co-assembled with some other, yet unidentified, molecules.
Trvalý link: http://hdl.handle.net/11104/0251164