Changes in the Sterol Composition of the Plasma Membrane Affect Membrane Potential, Salt Tolerance and the Activity of Multidrug Resistance Pumps in Saccharomyces cerevisiae

0448361 - FGÚ 2016 RIV US eng J - Článek v odborném periodiku
Kodedová, Marie - Sychrová, Hana
Changes in the Sterol Composition of the Plasma Membrane Affect Membrane Potential, Salt Tolerance and the Activity of Multidrug Resistance Pumps in Saccharomyces cerevisiae.
PLoS ONE. Roč. 10, č. 9 (2015), e0139306. ISSN 1932-6203. E-ISSN 1932-6203
Grant CEP: GA MŠMT(CZ) EE2.3.30.0025; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:67985823
Klíčová slova: Saccharomyces cerevisiae * ergosterol synthesis * multidrug resistance * membrane potential * diS-C3(3) assay
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 3.057, rok: 2015

We investigated the impact of the deletions of genes from the final steps in the biosynthesis of ergosterol (ERG6, ERG2, ERG3, ERG5, ERG4) on the physiological function of the Saccharomyces cerevisiae plasma membrane by a combination of biological tests and the diS-C3(3) fluorescence assay. Most of the erg mutants were more sensitive than the wild type to salt stress or cationic drugs, their susceptibilities were proportional to the hyperpolarization of their plasma membranes
Trvalý link: http://hdl.handle.net/11104/0250086