Capillary electrophoretic methods applied to the investigation of peptide complexes

0446783 - ÚOCHB 2016 RIV DE eng J - Článek v odborném periodiku
Štěpánová, Sille - Kašička, Václav
Capillary electrophoretic methods applied to the investigation of peptide complexes.
Journal of Separation Science. Roč. 38, č. 15 (2015), s. 2708-2721. ISSN 1615-9306. E-ISSN 1615-9314
Grant CEP: GA ČR(CZ) GA13-17224S
Institucionální podpora: RVO:61388963
Klíčová slova: affinity capillary electrophoresis * binding constants * non-covalent interactions * peptides * stability constants
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 2.741, rok: 2015

This article gives an overview of the applications of capillary electrophoretic methods to investigate the non-covalent interactions of peptides (peptide complexes) with variable middle- and high-molecular-mass receptors (ligands) as well as with small ions and molecules in the period 2007-2014. Different modes of capillary electrophoretic methods, such as mobility shift (vacancy) affinity capillary electrophoresis, multiple injection affinity capillary electrophoresis, partial filling affinity capillary electrophoresis, Hummel-Dryer method, vacancy peak method and (continuous) frontal analysis capillary electrophoresis, are briefly described and their applicability to determination of binding constants of peptide complexes is discussed. In addition, the detailed experimental conditions of individual applications and the values of binding constants of the particular peptide complexes are presented.
Trvalý link: http://hdl.handle.net/11104/0248790