Intracellular microRNA profiles form in the Xenopus laevis oocyte that may contribute to asymmetric cell division

0446657 - BTÚ 2016 RIV GB eng J - Článek v odborném periodiku
Šídová, Monika - Šindelka, Radek - Castoldi, M. - Benes, V. - Kubista, Mikael
Intracellular microRNA profiles form in the Xenopus laevis oocyte that may contribute to asymmetric cell division.
Scientific Reports. Roč. 5, č. 11157 (2015). ISSN 2045-2322. E-ISSN 2045-2322
Grant CEP: GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:86652036
Klíčová slova: VG1 MESSENGER-RNA * VEGETAL CORTEX * FROG OOCYTE
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 5.228, rok: 2015

Asymmetric distribution of fate determinants within cells is an essential biological strategy to prepare them for asymmetric division. In this work we measure the intracellular distribution of 12 maternal microRNAs (miRNA) along the animal-vegetal axis of the Xenopus laevis oocyte using qPCR tomography. We find the miRNAs have distinct intracellular profiles that resemble two out of the three profiles we previously observed for mRNAs. Our results suggest that miRNAs in addition to proteins and mRNAs may have asymmetric distribution within the oocyte and may contribute to asymmetric cell division as cell fate determinants.
Trvalý link: http://hdl.handle.net/11104/0248637