0446308 - BFÚ 2016 RIV NL eng J - Článek v odborném periodiku
Fojtová, Miloslava - Sýkorová, Eva - Najdekrová, Lucie - Polanská, P. - Zachová, D. - Vágnerová, Radka - Angelis, Karel - Fajkus, Jiří
Telomere dynamics in the lower plant Physcomitrella patens.
Plant Molecular Biology. Roč. 87, č. 6 (2015), s. 591-601. ISSN 0167-4412. E-ISSN 1573-5028
Grant CEP: GA ČR(CZ) GA13-06595S
Institucionální podpora: RVO:68081707
Klíčová slova: TERMINAL TRANSFERASE * ARABIDOPSIS-THALIANA * END-PROTECTION
Kód oboru RIV: BO - Biofyzika; EF - Botanika (UEB-Q)
Impakt faktor: 3.905, rok: 2015

A comparative approach in biology is needed to assess the universality of rules governing this discipline. In plant telomere research, most of the key principles were established based on studies in only single model plant, Arabidopsis thaliana. These principles include the absence of telomere shortening during plant development and the corresponding activity of telomerase in dividing (meristem) plant cells. Here we examine these principles in Physcomitrella patens as a representative of lower plants. To follow telomerase expression, we first characterize the gene coding for the telomerase reverse transcriptase subunit PpTERT in P. patens, for which only incomplete prediction has been available so far. In protonema cultures of P. patens, growing by filament apical cell division, the proportion of apical (dividing) cells was quantified and telomere length, telomerase expression and activity were determined. Our results show telomere stability and demonstrate proportionality of telomerase activity and expression with the number of apical cells.
Trvalý link: http://hdl.handle.net/11104/0248304