Characterisation of an unusual telomere motif (TTTTTTAGGG)(n) in the plant Cestrum elegans (Solanaceae), a species with a large genome

0446267 - BFÚ 2016 RIV GB eng J - Článek v odborném periodiku
Peška, Vratislav - Fajkus, Petr - Fojtová, Miloslava - Dvořáčková, Martina - Hapala, J. - Dvořáček, Vojtěch - Polanská, P. - Leitch, A. R. - Sýkorová, Eva - Fajkus, Jiří
Characterisation of an unusual telomere motif (TTTTTTAGGG)(n) in the plant Cestrum elegans (Solanaceae), a species with a large genome.
Plant Journal. Roč. 82, č. 4 (2015), s. 644-654. ISSN 0960-7412. E-ISSN 1365-313X
Grant CEP: GA ČR(CZ) GP13-10948P
Institucionální podpora: RVO:68081707
Klíčová slova: TERMINAL TRANSFERASE * SEQUENCING DATA * EVOLUTION
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 5.468, rok: 2015

The characterization of unusual telomere sequence sheds light on patterns of telomere evolution, maintenance and function. Plant species from the closely related genera Cestrum, Vestia and Sessea (family Solanaceae) lack known plant telomeric sequences. Here we characterize the telomere of Cestrum elegans, work that was a challenge because of its large genome size and few chromosomes (1C 9.76pg; n=8). We developed an approach that combines BAL31 digestion, which digests DNA from the ends and chromosome breaks, with next-generation sequencing (NGS), to generate data analysed in RepeatExplorer, designed for de novo repeats identification and quantification. We identify an unique repeat motif (TTTTTTAGGG)(n) in C.elegans, occurring in ca.30400 copies per haploid genome, averaging ca.1900 copies per telomere, and synthesized by telomerase. We demonstrate that the motif is synthesized by telomerase.
Trvalý link: http://hdl.handle.net/11104/0248258