Identification of individual biofilm-forming bacterial cells using Raman tweezers

0446098 - ÚPT 2016 RIV US eng J - Článek v odborném periodiku
Samek, Ota - Bernatová, Silvie - Ježek, Jan - Šiler, Martin - Šerý, Mojmír - Krzyžánek, Vladislav - Hrubanová, Kamila - Zemánek, Pavel - Holá, V. - Růžička, F.
Identification of individual biofilm-forming bacterial cells using Raman tweezers.
Journal of Biomedical Optics. Roč. 20, č. 5 (2015), 051038:1-6. ISSN 1083-3668. E-ISSN 1560-2281
Grant CEP: GA ČR GAP205/11/1687; GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01
Institucionální podpora: RVO:68081731
Klíčová slova: Raman tweezers * Staphylococcus epidermidis * biofilm
Kód oboru RIV: BH - Optika, masery a lasery
Impakt faktor: 2.556, rok: 2015

A method for in vitro identification of individual bacterial cells is presented. The method is based on a combination of optical tweezers for spatial trapping of individual bacterial cells and Raman microspectroscopy for acquisition of spectral "Raman fingerprints" obtained from the trapped cell. Here, Raman spectra were taken from the biofilm-forming cells without the influence of an extracellular matrix and were compared with biofilmnegative cells. Results of principal component analyses of Raman spectra enabled us to distinguish between the two strains of Staphylococcus epidermidis. Thus, we propose that Raman tweezers can become the technique of choice for a clearer understanding of the processes involved in bacterial biofilms which constitute a highly privileged way of life for bacteria, protected from the external environment.
Trvalý link: http://hdl.handle.net/11104/0248073