Magnetic beads-based electrochemical immunosensor for monitoring allergenic food proteins

0445033 - ÚMCH 2016 RIV US eng J - Článek v odborném periodiku
Čadková, M. - Metelka, R. - Holubová, L. - Horák, Daniel - Dvořáková, V. - Bílková, Z. - Korecká, L.
Magnetic beads-based electrochemical immunosensor for monitoring allergenic food proteins.
Analytical Biochemistry. Roč. 484, 1 September (2015), s. 4-8. ISSN 0003-2697. E-ISSN 1096-0309
Grant CEP: GA ČR GAP206/12/0381
Institucionální podpora: RVO:61389013
Klíčová slova: electrochemical immunosensor * magnetic particles * ovalbumin
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 2.243, rok: 2015

Screen-printed platinum electrodes as transducer and magnetic beads as solid phase were combined to develop a particle-based electrochemical immunosensor for monitoring the serious food allergen ovalbumin. The standard arrangement of enzyme-linked immunosorbent assay became the basis for designing the immunosensor. A sandwich-type immunocomplex was formed between magnetic particles functionalized with specific anti-ovalbumin immunoglobulin G and captured ovalbumin molecules, and secondary anti-ovalbumin antibodies conjugated with the enzyme horseradish peroxidase were subsequently added as label tag. The electrochemical signal proportional to the enzymatic reaction of horseradish peroxidase during the reduction of hydrogen peroxide with thionine as electron mediator was measured by linear sweep voltammetry. The newly established method of ovalbumin detection exhibits high sensitivity suitable for quantification in the range of 11 to 222 nM and a detection limit of 5 nM. Magnetic beads-based assay format using external magnets for rapid and simple separation has been proven to be an excellent basis for electrochemical detection and quantification of food allergens in highly complex sample matrices.
Trvalý link: http://hdl.handle.net/11104/0248146