Phylogenetic relatedness determined between antibiotic resistance and 16S rRNA genes in actinobacteria

0444561 - MBÚ 2016 RIV GB eng J - Článek v odborném periodiku
Ságová-Marečková, M. - Ulanová, Dana - Šanderová, P. - Omelka, M. - Kameník, Zdeněk - Olšovská, J. - Kopecký, J.
Phylogenetic relatedness determined between antibiotic resistance and 16S rRNA genes in actinobacteria.
BMC Microbiology. Roč. 15, APR 2015 (2015). ISSN 1471-2180. E-ISSN 1471-2180
Institucionální podpora: RVO:61388971
Klíčová slova: Actinobacteria * 16S rRNA diversity * Resistance genes
Kód oboru RIV: EH - Ekologie - společenstva
Impakt faktor: 2.581, rok: 2015

Distribution and evolutionary history of resistance genes in environmental actinobacteria provide information on intensity of antibiosis and evolution of specific secondary metabolic pathways at a given site. To this day, actinobacteria producing biologically active compounds were isolated mostly from soil but only a limited range of soil environments were commonly sampled. Consequently, soil remains an unexplored environment in search for novel producers and related evolutionary questions. Ninety actinobacteria strains isolated at contrasting soil sites were characterized phylogenetically by 16S rRNA gene, for presence of erm and ABC transporter resistance genes and antibiotic production. An analogous analysis was performed in silico with 246 and 31 strains from Integrated Microbial Genomes (JGI_IMG) database selected by the presence of ABC transporter genes and erm genes, respectively. In the isolates, distances of erm gene sequences were significantly correlated to phylogenetic distances based on 16S rRNA genes, while ABC transporter gene distances were not. The phylogenetic distance of isolates was significantly correlated to soil pH and organic matter content of isolation sites. In the analysis of JGI_IMG datasets the correlation between phylogeny of resistance genes and the strain phylogeny based on 16S rRNA genes or five housekeeping genes was observed for both the erm genes and ABC transporter genes in both actinobacteria and streptomycetes. However, in the analysis of sequences from genomes where both resistance genes occurred together the correlation was observed for both ABC transporter and erm genes in actinobacteria but in streptomycetes only in the erm gene.
Trvalý link: http://hdl.handle.net/11104/0257968