Cellular responses modulated by FGF-2 adsorbed on albumin/heparin layer-by-layer assemblies

0444496 - ÚMCH 2016 RIV US eng J - Článek v odborném periodiku
Kumorek, Marta M. - Kubies, Dana - Filová, Elena - Houska, Milan - Kasoju, Naresh - Mázl Chánová, Eliška - Matějka, Roman - Krýslová, Markéta - Bačáková, Lucie - Rypáček, František
Cellular responses modulated by FGF-2 adsorbed on albumin/heparin layer-by-layer assemblies.
PLoS ONE. Roč. 10, č. 5 (2015), e0125484_1-e0125484_23. ISSN 1932-6203. E-ISSN 1932-6203
Grant CEP: GA MŠMT(CZ) EE2.3.30.0029; GA ČR(CZ) GAP108/11/1857; GA ČR GPP108/12/P624; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:61389013 ; RVO:67985823
Klíčová slova: self-assembly * layer-by-layer * heparin
Kód oboru RIV: CD - Makromolekulární chemie; EI - Biotechnologie a bionika (FGU-C)
Impakt faktor: 3.057, rok: 2015

In a typical cell culture system, growth factors immobilized on the cell culture surfaces can serve as a reservoir of bio-signaling molecules, without the need to supplement them additionally into the culture medium. In this paper, we report on the fabrication of albumin/heparin (Alb/Hep) assemblies for controlled binding of basic fibroblast growth factor (FGF-2). The surfaces were constructed by layer-by-layer adsorption of polyelectrolytes albumin and heparin and were subsequently stabilized by covalent crosslinking with glutaraldehyde. An analysis of the surface morphology by atomic force microscopy showed that two Alb/Hep bilayers are required to cover the surface of substrate. The formation of the Alb/Hep assemblies was monitored by the surface plasmon resonance (SPR), the infrared multiinternal reflection spectroscopy (FTIR MIRS) and UV/VIS spectroscopy. The adsorption of FGF-2 on the cross-linked Alb/Hep was followed by SPR. The results revealed that FGF-2 binds to the Alb/Hep assembly in a dose and time-dependent manner up to the surface concentration of 120 ng/cm2. The bioactivity of the adsorbed FGF-2 was assessed in experiments in vitro, using calf pulmonary arterial endothelial cells (CPAE). CPAE cells could attach and proliferate on Alb/Hep surfaces. The adsorbed FGF-2 was bioactive and stimulated both the proliferation and the differentiation of CPAE cells. The improvement was more pronounced at a lower FGF-2 surface concentration (30 ng/cm2) than on surfaces with a higher concentration of FGF-2 (120 ng/cm2).
Trvalý link: http://hdl.handle.net/11104/0247014