alpha-L-Rhamnosyl-beta-D-glucosidase (Rutinosidase) from Aspergillus niger: Characterization and Synthetic Potential of a Novel Diglycosidase

0444488 - MBÚ 2016 RIV DE eng J - Článek v odborném periodiku
Šimčíková, Daniela - Kotík, Michael - Weignerová, Lenka - Halada, Petr - Pelantová, Helena - Adamcová, K. - Křen, Vladimír
alpha-L-Rhamnosyl-beta-D-glucosidase (Rutinosidase) from Aspergillus niger: Characterization and Synthetic Potential of a Novel Diglycosidase.
Advanced Synthesis & Catalysis. Roč. 357, č. 1 (2015), s. 107-117. ISSN 1615-4150. E-ISSN 1615-4169
Grant CEP: GA MŠMT(CZ) LD13042; GA MŠMT(CZ) 7E11011
Institucionální podpora: RVO:61388971
Klíčová slova: diglycosidase * enzyme catalysis * glycosides
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 6.453, rok: 2015

We report the first heterologous production of a fungal rutinosidase (6-O-alpha-L-rhamnopyranosyl-beta-d-glucopyranosidase) in Pichia pastoris. The recombinant rutinosidase was purified from the culture medium to apparent homogeneity and biochemically characterized. The enzyme reacts with rutin and cleaves the glycosidic linkage between the disaccharide rutinose and the aglycone. Furthermore, it exhibits high transglycosylation activity, transferring rutinose from rutin as a glycosyl donor onto various alcohols and phenols. The utility of the recombinant rutinosidase was demonstrated by its use for the synthesis of a broad spectrum of rutinosides of primary (saturated and unsaturated), secondary, acyclic and phenolic alcohols as well as for the preparation of free rutinose. Moreover, the alpha-L-rhamnosidase-catalyzed synthesis of a chromogenic substrate for a rutinosidase assay - para-nitrophenyl beta-rutinoside - is described.
Trvalý link: http://hdl.handle.net/11104/0247003